| The Rev. Dr. Theophilus Stork | 1859-1860 |
| The Rev. Dr. James Allen Brown | 1860-1861 |
| Robert Garlington (Interim) | 1861 |
| The Rev. Dr. Josiah P. Smeltzer | 1861-1877 |
| The Rev. Dr. George W. Holland | 1877-1895 |
| Dr. George B. Cromer | 1895-1904 |
| The Rev. Dr. James A. B. Scherer | 1904-1908 |
| The Rev. Dr. J. Henry Harms | 1908-1918 |
| Dr. Sidney J. Derrick | 1918-1930 |
| Dr. James C. Kinard | 1930-1954 |
| Dr. Christopher A. Kaufman | 1954-1960 |
| Dr. Conrad B. Park (Acting) | 1960 |
| Dr. A. G. D. Wiles | 1960-1971 |
| Dr. Fredric B. Irvin | 1971-1975 |
| Dr. Glenn E. Whitesides | 1975-1984 |
| Dr. John S. Ammarell (Interim) | 1984 |
| Dr. Paul F. Tillquist | Jan. 1985 |
| Dr. John S. Ammarell | Feb. 1985 - 1986 |
| Dr. Hubert H. Setzler, Jr. | 1986-1992 |
| The Rev. Dr. Raymond M. Bost | 1992-1995 |
| Dr. Peter L. French | 1995-1999 |
| Dr. John H. Hudgens (Interim) | 1999-2000 |
| Dr. Mitchell M. Zais | 2000-2010 |
| Dr. John H. Hudgens (Acting) | Spring 2010 |
| Dr. V. Scott Koerwer | 2010-2011 |
| Dr. John H. Hudgens (Acting) | 2011-2012 |
| Dr. Maurice W. Scherrens | 2012-2025 |
| Dr. David Harpool (Transitional) | 2025 - present |
